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Charles, B.G. et al.

Measurement of gliclazide in plasma by radial compression reversed-phase liquid chromatography.

We describe a rapid, specific, and precise analysis for gliclazide in plasma by radial compression, reversed-phase, "high-performance" liquid chromatography. Gliclazide and the internal standard, 3-chlorogliclazide, are eluted after 4.4 and 6.8 min, respectively. Only 100 microL of plasma and minimal sample workup are required. The limit of detection for gliclazide in plasma is 0.5 mg/L (1.55 mumol/L) at 229 nm. Precision (CV) of the assay for 10 and 1 mg of gliclazide per litre is 2.1% and 6.4%, respectively.[1]

References

Measurement of gliclazide in plasma by radial compression reversed-phase liquid chromatography. Charles, B.G., Ravenscroft, P.J. Clin. Chem. (1984) [Pubmed]

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