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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A comparison of fluorescamine and o-phthaldialdehyde as effective blocking reagents in protein sequence analyses by the Beckman sequencer.

Use of o-phthaldialdehyde to chemically reduce the newly generated amino termini responsible for the progressively increasing background during an extended amino acid sequence analysis in a liquid phase sequencer has been described. The results have been compared with Fluram blocking using apomyoglobin and rabbit C-reactive protein as standard and unknown samples, respectively.[1]

References

  1. A comparison of fluorescamine and o-phthaldialdehyde as effective blocking reagents in protein sequence analyses by the Beckman sequencer. Bhown, A.S., Cornelius, T.W., Volanakis, J.E., Bennett, J.C. Anal. Biochem. (1983) [Pubmed]
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