Disease relevance of SE0989

• A novel peptide screened by phage display can mimic TRAP antigen epitope against Staphylococcus aureus infections [1].
• Plasmid profiles of cultures of Staphylococcus epidermidis obtained by agarose gel electrophoresis were used, in conjunction with phage typing, biotyping, and antibiograms, in epidemiologic studies of infections in neonates and patients with cancer [2].
• Sixteen of the 90 phages were selected for phage typing of S. epidermidis [3].
• Plasmid profiles, phage typing, antibiograms, and biotyping were used to characterize Staphylococcus epidermidis isolated from multiple cultures of blood of four patients with prosthetic valve endocarditis [4].
• Conjugate-resistant E. coli mutants showed the absence of some iron-regulated outer membrane proteins in gel electrophoresis profiles and in specific phage or colicin sensitivity tests, implying that the drugs used outer membrane receptors of ferric complexes to get into cells [5].

High impact information on SE0989

• In individual patients, prolonged episodes of septicemia were confirmed by multiple blood culture isolates of S. epidermidis, identical in antibiotic resistance pattern, phage type, and biotype [6].
• Transduction by S. epidermidis phage 71 was used to prove the genetic linkage of transposon insertions and altered phenotypes [7].
• To further characterize the Fg-binding activity, a shotgun phage display library covering the S. epidermidis chromosome was constructed [8].
• Linkage of transposon insertions of mutants M10 and M11 with the altered phenotype was demonstrated by phage transduction, whereas the several other mutants apparently represented spontaneous variants [9].
• Our observations provide evidence for the conjugal transfer of some staphylococcal plasmids, apparently independent of the presence of phage [10].

Biological context of SE0989

• Statistical analysis of data obtained by typing showed no predominance of any endemic clone: the strains colonizing the immunocompromised patients and isolated from staff and inanimated environment differed from each other in biotype, phage pattern, antibiotic susceptibility, slime production and/or plasmid profile [11].
• The Staphylococcus epidermidis phage group, although morphologically homogeneous, was heterogeneous by DNA/DNA hybridization (27 to 100% homology) and seroneutralization kinetics [12].
• In combination with phage transduction, 1457c x pTV1ts will be a useful tool facilitating the study of bacterial determinants of the pathogenicity of S. epidermidis [13].
• In order to affirm the clonal origins of these isolates, six phenotype (biotype, anti-biotype, serotype, phage type), and genotype (random amplified polymorphic DNA, pulsed field gel electrophoresis) methods were used [14].

Anatomical context of SE0989

• Cultures from patients with middle ear infections were also classified by this system and phage typed [15].
• Isolates of coagulase negative staphylococci from the middle ear, external ear and posterior aural skin were evaluated in regard to antibiotic sensitivity, biochemical tests and phage typing [16].

Associations of SE0989 with chemical compounds

• METHODS--Microbiological typing was done by biotyping, phage typing, and polyacrylamide gel electrophoresis of radiolabelled bacterial proteins (radioPAGE method) [17].
• Gentamicin-resistant isolates were characterized by biotyping, antibiotic sensitivity pattern and phage-typing [18].
• Antibiotic susceptibility testing, phage-typing, bio-typing, and test for Tween-80-splitting enzyme were used to characterize 132 isolates from nose swabs [19].
• Approximately 10(-5) transductant colonies per phage input were obtained and ultraviolet kinetics indicated that Pc was plasmid carried [20].

Analytical, diagnostic and therapeutic context of SE0989

• The present status of phage typing S. epidermidis and the work needed to obtain a set of typing phages for epidemiological studies of infections by this organism are discussed [21].
• The total DNA restriction endonuclease analysis (REA) was processed along with the following conventional markers: biotyping, serotyping, phage typing, antibiotic susceptibility profiles, and plasmid profiles [22].

References