Disease relevance of PVALB

• Molecular cloning of the thymus-specific parvalbumin known as avian thymic hormone: isolation of a full length cDNA and expression of the recombinant protein in Escherichia coli [1].

High impact information on PVALB

• However, the CPV3 content of chicken thymus tissue (120 micrograms/g tissue) is 4 times lower than that of ATH (500 micrograms/g tissue) [2].
• Avian thymic hormone exhibits a pI = 4.35 when subjected to isoelectric focusing through polyacrylamide gels [3].
• Confocal microscopy of the stained sections reveals that ATH and CPV3 are both confined to the thymic cortex and that they are frequently coexpressed by a subset of epithelial cells [4].
• Isoform-specific monoclonal antibodies against ATH and CPV3 were labeled with fluorescein and Cy5, respectively, and then used to probe paraffin sections of chicken thymus tissue [4].
• Avian thymic hormone (ATH) is a parvalbumin produced by epithelial cells in the thymic cortex of chickens and circulates in the blood on a 5-day cycle [5].

Biological context of PVALB

• The incomplete amino acid sequence of the protein identified as avian thymic hormone was recently reported [Brewer et al. (1989), Biochem. Biophys. Res. Commun. 160, 11555-1161], and a very high degree of homology to the parvalbumins was apparent [6].
• Most notably, the nucleotide sequence indicates that the CD site of avian thymic hormone is a typical parvalbumin CD site [6].

Anatomical context of PVALB

• Partial nucleotide sequence of the parvalbumin from chicken thymus designated "avian thymic hormone" [6].
• The complete amino acid sequence of 'avian thymic hormone' (ATH), a protein from thymus tissue that appears to promote immune maturation in chicken bone marrow cells in culture, is presented [7].
• Because ATH targets gammadelta T cells, the data implicate participation of donor gammadelta T cells in the acute GVHR [5].
• Hybridoma screening by enzyme-linked immunosorbent assay (ELISA) led to the culture, purification, and characterization of an hybridoma, designated 4A6, which secretes IgG1 that is specific for ATH [8].

Associations of PVALB with chemical compounds

• However, in contrast to ATH isolated from chicken thymus tissue, the N-terminal alanine of recombinant ATH is unacetylated [1].

Other interactions of PVALB

• The amino acid sequence of avian thymic hormone, a parvalbumin [7].

Analytical, diagnostic and therapeutic context of PVALB

• The sequence was obtained from sequences of ATH peptides isolated by HPLC after tryptic, chymotryptic, peptic or S aureus V8 protease digestions [7].
• Avian thymic hormone and chicken (muscle) parvalbumin are distinct proteins: isolation of a muscle parvalbumin cDNA fragment by PCR [9].
• An avian thymic hormone, originally designated the T1-antigen, was purified from chicken thymus by Sephadex G-75-40 chromatography and affinity chromatography, following enrichment by heat and pH treatments [10].

References