High impact information on Apex2

• Apex2 consists of six exons and is flanked on the 3' end by Alas2 on X chromosome 63 [1].
• Immunoelectron microscopy revealed that APEX2 is also localized in the mitochondria of Apex2(+) cells [1].
• We disrupted Apex2 in embryonic stem cells derived from a male mouse, and a 55-kDa APEX2 protein was detected in the nuclei of Apex2(+) but not Apex2-disrupted cells [1].
• The level of Apex2 mRNA was high in the thymus, bone marrow, spleen, and kidney in adult mice [1].
• In serum-stimulated BALB/c 3T3 cells, the level of Apex2 mRNA was transiently increased and the level of APEX2 reached a maximum in the late S phase, thus indicating that APEX2 may participate in postreplicative base excision repair [1].

Biological context of Apex2

• Much less is known about Ape2, but its importance is emphasized by the growth retardation and dyshematopoiesis accompanied by G2/M arrest phenotype of the APE2-null mice [2].
• Mutation of the active-site residue Asp 277 to Ala in Ape2 inactivates all these activities [2].

References