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Gene Review

YIP1  -  Yip1p

Saccharomyces cerevisiae S288c

Synonyms: Protein transport protein YIP1, YGR172C, YPT-interacting protein 1
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High impact information on YIP1

  • Finally, ordering experiments show that preincubation of ER membranes with COPII proteins decreases sensitivity to anti-Yip1p antibodies, indicating an early requirement for Yip1p in vesicle formation [1].
  • A role for Yip1p in COPII vesicle biogenesis [1].
  • Using a cell-free assay that recapitulates protein transport from the ER to the Golgi complex, we find that affinity-purified antibodies directed against the hydrophilic amino terminus of Yip1p potently inhibit transport [1].
  • We encountered Yip1p as a constituent of ER-derived transport vesicles, leading us to hypothesize a direct role for this protein in transport through the early secretory pathway [1].
  • Specific binding to a novel and essential Golgi membrane protein (Yip1p) functionally links the transport GTPases Ypt1p and Ypt31p [2].

Biological context of YIP1

  • The exact role of Yip1p is unclear; YIP1 is an essential gene and defective alleles severely disrupt membrane transport and inhibit ER vesicle budding [3].
  • Yip1p is the first identified Rab-interacting membrane protein and the founder member of the YIP1 family, with both orthologs and paralogs found in all eukaryotic genomes [3].
  • We demonstrate that the Yip1p-related proteins possess several features: (i) they have a common overall domain topology, (ii) they are capable of biochemical interaction with a variety of Rab proteins in a manner dependent on C-terminal prenylation, and (iii) they share an ability to physically associate with other members of the YIP1 family [4].
  • Like the two GTPases, Yip1p is essential for yeast cell viability and, according to subcellular fractionation and indirect immunofluorescence, is located to Golgi membranes at steady state [2].
  • Mutant cells depleted of Yip1p and conditionally lethal yip1 mutants at the non-permissive temperature massively accumulate endoplasmic reticulum membranes and display aberrations in protein secretion and glycosylation of secreted invertase [2].

Anatomical context of YIP1

  • The biochemical capability of YIP1 family proteins suggests a possible role in the cycle of physical localization of Rab proteins between their cognate membranes and the cytosol [5].
  • To block fusion, anti-Yip1p or anti-Yif1p antibodies must be added before vesicles bud from the endoplasmic reticulum (ER) [6].
  • Yip1p has the ability to physically interact with Rab proteins and the nature of this interaction has led to suggestions that Yip1p may function in the process by which Rab proteins translocate between cytosol and membranes [3].

Physical interactions of YIP1

  • Yop1p is a membrane protein with a hydrophilic region at its N terminus through which it interacts specifically with the cytosolic domain of Yip1p [7].

Other interactions of YIP1

  • The yeast (Saccharomyces cerevisiae) HVA22 homolog, Yop1p, has been shown to interact with the GTPase-interacting protein, Yip1p [8].
  • Yos1p is a novel subunit of the Yip1p-Yif1p complex and is required for transport between the endoplasmic reticulum and the Golgi complex [9].
  • In this study we identify Yif1p, and two unknown open reading frames, Ygl198p and Ygl161p, which we term Yip4p and Yip5p, as Yip1p-related sequences [4].
  • Yip1p function requires Rab-GDI and Rab proteins, and several mutations that abrogate Yip1p function lack Rab-interacting capability [3].
  • The family includes yeast Yip1p, Yip4p, Yip5p, and Yif1p, and also their plant, insects, nematode, and mammalian homologues, suggesting their conserved function in a broad range of species [10].

Analytical, diagnostic and therapeutic context of YIP1


  1. A role for Yip1p in COPII vesicle biogenesis. Heidtman, M., Chen, C.Z., Collins, R.N., Barlowe, C. J. Cell Biol. (2003) [Pubmed]
  2. Specific binding to a novel and essential Golgi membrane protein (Yip1p) functionally links the transport GTPases Ypt1p and Ypt31p. Yang, X., Matern, H.T., Gallwitz, D. EMBO J. (1998) [Pubmed]
  3. Genetic analysis of yeast Yip1p function reveals a requirement for Golgi-localized rab proteins and rab-Guanine nucleotide dissociation inhibitor. Chen, C.Z., Calero, M., DeRegis, C.J., Heidtman, M., Barlowe, C., Collins, R.N. Genetics (2004) [Pubmed]
  4. Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors. Calero, M., Winand, N.J., Collins, R.N. FEBS Lett. (2002) [Pubmed]
  5. Insights into biological functions across species: examining the role of Rab proteins in YIP1 family function. Chen, C.Z., Collins, R.N. Biochem. Soc. Trans. (2005) [Pubmed]
  6. The Yip1p.Yif1p complex is required for the fusion competence of endoplasmic reticulum-derived vesicles. Barrowman, J., Wang, W., Zhang, Y., Ferro-Novick, S. J. Biol. Chem. (2003) [Pubmed]
  7. Yop1p, the yeast homolog of the polyposis locus protein 1, interacts with Yip1p and negatively regulates cell growth. Calero, M., Whittaker, G.R., Collins, R.N. J. Biol. Chem. (2001) [Pubmed]
  8. Function of a plant stress-induced gene, HVA22. Synthetic enhancement screen with its yeast homolog reveals its role in vesicular traffic. Brands, A., Ho, T.H. Plant Physiol. (2002) [Pubmed]
  9. Yos1p is a novel subunit of the Yip1p-Yif1p complex and is required for transport between the endoplasmic reticulum and the Golgi complex. Heidtman, M., Chen, C.Z., Collins, R.N., Barlowe, C. Mol. Biol. Cell (2005) [Pubmed]
  10. Identification of a five-pass transmembrane protein family localizing in the Golgi apparatus and the ER. Shakoori, A., Fujii, G., Yoshimura, S., Kitamura, M., Nakayama, K., Ito, T., Ohno, H., Nakamura, N. Biochem. Biophys. Res. Commun. (2003) [Pubmed]
  11. Human Yip1A specifies the localization of Yif1 to the Golgi apparatus. Jin, C., Zhang, Y., Zhu, H., Ahmed, K., Fu, C., Yao, X. Biochem. Biophys. Res. Commun. (2005) [Pubmed]
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