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Chemical Compound Review: 

Purpald     4-amino-5-hydrazinyl-2H- 1,2,4-triazole-3...

Synonyms: Purpald(R), SureCN37997, ACMC-1BYMH, AG-E-24658, ANW-43179, ...
 
 
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High impact information on Purpald

  • For SG-containing PS, conversion of SG to UG with 25 micro;L of 0.3 N NaOH, 1 h at room temperature for de-O-acetylation followed by 25 microL of 0.6 M H2SO4, 1 h at 80 degrees C for acid hydrolysis of PS precedes the periodate treatment in the purpald assay [1].
  • The quantity of H2O2 is determined by the absorbance at 550 nm in the presence of catalase and 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (AHMT) [2].
  • Catalase was determined according to the method of Johansson and Borg with use of Purpald [3].

References

  1. Quantification of bacterial polysaccharides by the purpald assay: measurement of periodate-generated formaldehyde from glycol in the repeating unit. Lee, C.H., Frasch, C.E. Anal. Biochem. (2001) [Pubmed]
  2. A new colorimetric method for the determination of free fatty acids with acyl-CoA synthetase and acyl-CoA oxidase. Hosaka, K., Kikuchi, T., Mitsuhida, N., Kawaguchi, A. J. Biochem. (1981) [Pubmed]
  3. The effect of repeated administration of selected benzene bromoderivatives on the liver catalase activity in rats. Czerski, J., Szymańska, J.A. International journal of occupational medicine and environmental health. (2005) [Pubmed]
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