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Chemical Compound Review:

MESG (2R,3R,4R,5R)-2-(2-amino-7- methyl-6...

Synonyms: AC1MHZG7, 55727-10-1, 6-Mercapto-7-methylguanosine

This record was replaced with 3035491.

Lloyd, A.J. et al., Upson, R.H. et al., Silva, R.G. et al.

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High impact information on 2-amino-9-[(2R,3R,4R,5R)-3,4-dihydroxy-5-methylol-tetrahydrofuran-2-yl]-7-methyl-3H-purine-6-thione

Pyrophosphate release is coupled to inorganic pyrophosphatase, to generate phosphate, which in turn is used as the substrate of purine nucleoside phosphorylase to catalyze the N-glycosidic cleavage of 2-amino 6-mercapto 7-methylpurine ribonucleoside [1].
The phosphate is then detected by the conversion of 2-amino-6-mercapto-7-methylpurine ribonucleoside to 2-amino-6-mercapto-7-methylpurine by purine nucleoside phosphorylase [M.R. Webb (1992) Proc. Natl. Acad. Sci. USA 89, 4884-4887] [2].
Here we investigated the presence of PNP in CSF of rats using: i) a specific chromophoric analogue of nucleosides, 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG), and ii) an inhibitor of PNP activity, immucillin-H [3].

References

A broadly applicable continuous spectrophotometric assay for measuring aminoacyl-tRNA synthetase activity. Lloyd, A.J., Thomann, H.U., Ibba, M., Söll, D. Nucleic Acids Res. (1995) [Pubmed]
A spectrophotometric method to measure enzymatic activity in reactions that generate inorganic pyrophosphate. Upson, R.H., Haugland, R.P., Malekzadeh, M.N., Haugland, R.P. Anal. Biochem. (1996) [Pubmed]
Purine nucleoside phosphorylase activity in rat cerebrospinal fluid. Silva, R.G., Santos, D.S., Basso, L.A., Oses, J.P., Wofchuk, S., Portela, L.V., Souza, D.O. Neurochem. Res. (2004) [Pubmed]

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