High impact information on APH1A

• Only the combined overexpression of PS1 and NCT together with APH-1a G122D facilitated the formation of a fully active gamma-secretase complex [1].
• We conclude that cooperative effects may stabilize a trim-eric complex of APH-1a G122D together with PS1 and NCT [1].
• Under these conditions, APH-1a G122D supported the production of normal amounts of Abeta [1].
• Here we show that both APH-1a splice forms and APH-1b are expressed in peripheral and neuronal cells [2].
• Using the APH-1 protein encoded on human chromosome 1 (APH-1(1)L; also known as APH-1a) as an archetype, we report here that endogenous forms of APH-1 are predominantly expressed in intracellular membrane compartments, including the endoplasmic reticulum and cis-Golgi [3].

Biological context of APH1A

• Two homologous genes of APH-1 exist in humans, APH-1a and APH-1b, and alternative splicing of the former generates two isoforms, APH-1aS and APH-1aL [4].
• Here we characterized the 5'-flanking region of the human APH-1A gene and identified a 271-bp fragment containing the transcription initiation site for the promoter activity [5].

References