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Gene Review:

amyA - alpha-amylase

Escherichia coli O157:H7 str. EDL933

Mertens, J.A. et al., Horinouchi, S. et al., Bahl, H. et al., Wang, N. et al., Mijts, B.N. et al.

Mertens, Skory, Ibrahim,

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Disease relevance of amyA

The nucleotide sequence of the alpha-amylase gene (amyA) from Clostridium thermosulfurogenes EM1 cloned in Escherichia coli was determined [1].
The amyA gene was expressed in E. coli as a hexahistidine-tagged enzyme and purified [2].

High impact information on amyA

Three plasmid vectors have been created using either the glucoamylase A (amyA), pyruvate decarboxylase (pdcA), or phosphoglycerate kinase (pgk1) promoters to drive expression of heterologous proteins [3].
The codon usage in the amyA, amyB and amyC genes was highly biased, which reflects the fact that the guanine-plus-cytosine (G + C) content of DNA of D. thermophilum is 29 mol% [4].
The gene amyA consists of 1764 base pairs and was predicted to encode a 587-amino acid protein encompassing a 31-amino acid signal peptide [5].

References

alpha-Amylase of Clostridium thermosulfurogenes EM1: nucleotide sequence of the gene, processing of the enzyme, and comparison of other alpha-amylases. Bahl, H., Burchhardt, G., Spreinat, A., Haeckel, K., Wienecke, A., Schmidt, B., Antranikian, G. Appl. Environ. Microbiol. (1991) [Pubmed]
Cloning, sequencing and expression of an alpha-amylase gene, amyA, from the thermophilic halophile Halothermothrix orenii and purification and biochemical characterization of the recombinant enzyme. Mijts, B.N., Patel, B.K. Microbiology (Reading, Engl.) (2002) [Pubmed]
Plasmids for expression of heterologous proteins in Rhizopus oryzae. Mertens, J.A., Skory, C.D., Ibrahim, A.S. Arch. Microbiol. (2006) [Pubmed]
Cloning and expression in Escherichia coli of two additional amylase genes of a strictly anaerobic thermophile, Dictyoglomus thermophilum, and their nucleotide sequences with extremely low guanine-plus-cytosine contents. Horinouchi, S., Fukusumi, S., Ohshima, T., Beppu, T. Eur. J. Biochem. (1988) [Pubmed]
Gene cloning and characterization of a novel alpha-amylase from alkaliphilic Alkalimonas amylolytica. Wang, N., Zhang, Y., Wang, Q., Liu, J., Wang, H., Xue, Y., Ma, Y. Biotechnology journal (2006) [Pubmed]

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