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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
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Disease relevance of Plasmids

  • Fragments of 5S DNA produced by partial digestion with Hind III and containing 1, 4, and 5 repeating units have been inserted at the single Hind III site of the tetracycline-resistance plasmid, pSC101, and the hybrid plasmids cloned in E. coli [1].
  • The first method uses lambda DNA as the target of transposition and a plasmid containing the ends of Mu DNA and an ampicillin-resistance gene as the donor; after the reaction, in vitro lambda packaging allows the scoring of ampr transducing phages generated by transposition [2].
  • Investigation of a 4.9-fold increase in Salmonella newport isolations from Californians in 1985 showed that 87 percent of the isolates had an unusual antimicrobial-resistance pattern (including chloramphenicol resistance) and a single, identical plasmid [3].
  • Multidrug resistance in Yersinia pestis mediated by a transferable plasmid [4].
  • The demonstration of sequence-specific binding at multiple loci suggests that EBNA-1 has pleiotropic functions, which may include control of copy number and segregation of the EBV plasmids as well as initiation of replication [5].

Psychiatry related information on Plasmids

  • In none of the cases did the presence of the plasmid in the strains change either the initial rate of incorporation of [3H]thymidine triphosphate into acid-soluble material or the subsequent degradation of the template at longer reaction times [6].
  • OBJECTIVE: To test the safety of a single intracavernous injection of a plasmid vector (hMaxi-K) that expresses the hSlo gene, that encodes the alpha-subunit of the Maxi-K channel, for the treatment of erectile dysfunction (ED) [7].

High impact information on Plasmids


Chemical compound and disease context of Plasmids


Biological context of Plasmids

  • Their growth, unlike that of the cells containing ras alone, was not inhibited by transfection with plasmids encoding wild-type human p53 [17].
  • The promoters from the str and spc operons of ribosomal proteins from E. coli were inserted into the Hind II cleavage site of mini-Col E1 (pVH51) plasmid [18].
  • Only strain Paris contains a type V secretion system, and its Lvh type IV secretion system is encoded by a 36-kb region that is either carried on a multicopy plasmid or integrated into the chromosome [19].
  • The isolation of the two hybrid plasmids 56H8 and 132E3, which contain D. melanogaster (Dm) DNA sequences complementary to the mRNA coding for the 70,000 dalton heat shock protein, has been reported (Schedl et al., 1978) [20].
  • In vitro enzymatic alteration of plasmid phenotype and in vitro construction of recombinant plasmids containing genetic information derived from the plasmid pSC101 have been used to investigate the mechanism of function of tetracycline resistance determined by the plasmid pSC101 [21].

Anatomical context of Plasmids

  • Pre-B and pre-T cell lines from mutant mice with severe combined immune deficiency (scid mice) were transfected with plasmids that contained recombination signal sequences of antigen receptor gene elements (V, D, and J) [22].
  • A plasmid containing the complete transcription unit of the desmin gene was transfected into hamster lens cells and into human epithelial (HeLa) cells [23].
  • A nuclear extract from Xenopus oocytes is capable of supporting transcription of the tRNATrp gene contained on plasmid pBR313 [24].
  • A plasmid, designated pMK, containing the structural gene for thymidine kinase from herpes simplex virus (HSV) fused to the promoter/regulatory region of the mouse metallothionein-I gene, was injected into the pronucleus of fertilized one-cell mouse eggs; the eggs were subsequently reimplanted into the oviducts of pseudopregnant mice [25].
  • A high percentage of transgenic mice developing from eggs microinjected with plasmids containing the SV40 early region genes and a metallothionein fusion gene develop tumors within the choroid plexus [26].

Associations of Plasmids with chemical compounds

  • We describe a novel type of transposon in the tetracycline resistance plasmid pYM103, a derivative of pSC101 carrying a single copy of an insertion element IS102 [27].
  • We have inserted sheared embryonic D. melanogaster DNA by the poly(dA-dt) connector method (Lobban and Kaiser, 1973) into the R1 restriction site of the ampicillin-resistant plasmid pSF2124 (So, Gill and Falkow, 1975) [28].
  • Cells bearing these chimeric plasmids exhibited much higher levels of galactokinase than did cells bearing plasmids wherein the galactose promoter was fused to galK [29].
  • To study the function of the genes of the T-DNA of the nopaline Ti plasmid, pTiC58, a collection of mutants was isolated so that T-DNA genes are inactivated either separately or in various combinations [30].
  • We show here that a plasmid containing a cloned gene coding for a yeast leucine tRNA comes under developmental control when injected into cleaving eggs [31].

Gene context of Plasmids

  • Examination of the linking number distributions of plasmids bearing the inducible promoters of GAL1 and GAL10 genes indicates that the generation of supercoiled domains of opposite signs is related to transcription [32].
  • Moreover, GAL4 fragments bearing these 30 amino acids, when expressed from a strong promoter on multicopy plasmids, free the endogenous GAL4 from inhibition by GAL80 [33].
  • Two RNA-mediated recombination events were detected: homologous recombination between the cDNA and plasmid his3 sequences, resulting in intron loss, and insertion of the cDNA into the chromosome in the absence of HIS3 homology [34].
  • Four distinct forms of the excised actin intron are found in poly(A)- RNA from yeast carrying this transcription unit on a multicopy plasmid [35].
  • We have developed an in vitro system utilizing yeast cell-free extracts to catalyze recombination events between homologous plasmids containing different mutant alleles of the Tet or ARG4 genes [36].

Analytical, diagnostic and therapeutic context of Plasmids


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  2. In vitro transposition of bacteriophage Mu: a biochemical approach to a novel replication reaction. Mizuuchi, K. Cell (1983) [Pubmed]
  3. Chloramphenicol-resistant Salmonella newport traced through hamburger to dairy farms. A major persisting source of human salmonellosis in California. Spika, J.S., Waterman, S.H., Hoo, G.W., St Louis, M.E., Pacer, R.E., James, S.M., Bissett, M.L., Mayer, L.W., Chiu, J.Y., Hall, B. N. Engl. J. Med. (1987) [Pubmed]
  4. Multidrug resistance in Yersinia pestis mediated by a transferable plasmid. Galimand, M., Guiyoule, A., Gerbaud, G., Rasoamanana, B., Chanteau, S., Carniel, E., Courvalin, P. N. Engl. J. Med. (1997) [Pubmed]
  5. Sequence-specific DNA binding of the Epstein-Barr virus nuclear antigen (EBNA-1) to clustered sites in the plasmid maintenance region. Rawlins, D.R., Milman, G., Hayward, S.D., Hayward, G.S. Cell (1985) [Pubmed]
  6. The effects of the ultraviolet-protecting plasmids pKM101 and R205 on DNA polymerase I activity in Escherichia coli K-12. Kronish, J.W., Walker, G.C. Mutat. Res. (1979) [Pubmed]
  7. The first human trial for gene transfer therapy for the treatment of erectile dysfunction: preliminary results. Melman, A., Bar-Chama, N., McCullough, A., Davies, K., Christ, G. Eur. Urol. (2005) [Pubmed]
  8. Polyadenylation of mRNA in prokaryotes. Sarkar, N. Annu. Rev. Biochem. (1997) [Pubmed]
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  10. Fusion of NUP214 to ABL1 on amplified episomes in T-cell acute lymphoblastic leukemia. Graux, C., Cools, J., Melotte, C., Quentmeier, H., Ferrando, A., Levine, R., Vermeesch, J.R., Stul, M., Dutta, B., Boeckx, N., Bosly, A., Heimann, P., Uyttebroeck, A., Mentens, N., Somers, R., MacLeod, R.A., Drexler, H.G., Look, A.T., Gilliland, D.G., Michaux, L., Vandenberghe, P., Wlodarska, I., Marynen, P., Hagemeijer, A. Nat. Genet. (2004) [Pubmed]
  11. Bacterial mitotic machineries. Gerdes, K., Møller-Jensen, J., Ebersbach, G., Kruse, T., Nordström, K. Cell (2004) [Pubmed]
  12. Stimulation of a Chlamydomonas chloroplast promoter by novobiocin in situ and in E. coli implies regulation by torsional stress in the chloroplast DNA. Thompson, R.J., Mosig, G. Cell (1987) [Pubmed]
  13. Drug-resistant Salmonella from animals fed antimicrobials. Holmberg, S.D., Osterholm, M.T., Senger, K.A., Cohen, M.L. N. Engl. J. Med. (1984) [Pubmed]
  14. Failure of oral trimethoprim-sulfamethoxazole prophylaxis in acute leukemia: isolation of resistant plasmids from strains of Enterobacteriaceae causing bacteremia. Wilson, J.M., Guiney, D.G. N. Engl. J. Med. (1982) [Pubmed]
  15. Wide ranging plasmid bearing the Pseudomonas aeruginosa tryptophan synthase genes. Hedges, R.W., Jacob, A.E., Crawford, I.P. Nature (1977) [Pubmed]
  16. Rabies virus glycoprotein analogs: biosynthesis in Escherichia coli. Yelverton, E., Norton, S., Obijeski, J.F., Goeddel, D.V. Science (1983) [Pubmed]
  17. Ras-induced hyperplasia occurs with mutation of p53, but activated ras and myc together can induce carcinoma without p53 mutation. Lu, X., Park, S.H., Thompson, T.C., Lane, D.P. Cell (1992) [Pubmed]
  18. Isolation and characterization of stable hybrid mRNA molecules transcribed from ribosomal protein promoters in E. coli. Ikemura, T., Itoh, S., Post, L.E., Nomura, M. Cell (1979) [Pubmed]
  19. Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity. Cazalet, C., Rusniok, C., Brüggemann, H., Zidane, N., Magnier, A., Ma, L., Tichit, M., Jarraud, S., Bouchier, C., Vandenesch, F., Kunst, F., Etienne, J., Glaser, P., Buchrieser, C. Nat. Genet. (2004) [Pubmed]
  20. Physical map of two D. melanogaster DNA segments containing sequences coding for the 70,000 dalton heat shock protein. Moran, L., Mirault, M.E., Tissières, A., Lis, J., Schedl, P., Artavanis-Tsakonas, S., Gehring, W.J. Cell (1979) [Pubmed]
  21. On the nature of tetracycline resistance controlled by the plasmid pSC101. Tait, R.C., Boyer, H.W. Cell (1978) [Pubmed]
  22. The defect in murine severe combined immune deficiency: joining of signal sequences but not coding segments in V(D)J recombination. Lieber, M.R., Hesse, J.E., Lewis, S., Bosma, G.C., Rosenberg, N., Mizuuchi, K., Bosma, M.J., Gellert, M. Cell (1988) [Pubmed]
  23. Characterization of the hamster desmin gene: expression and formation of desmin filaments in nonmuscle cells after gene transfer. Quax, W., van den Broek, L., Egberts, W.V., Ramaekers, F., Bloemendal, H. Cell (1985) [Pubmed]
  24. In vitro transcription and processing of a yeast tRNA gene containing an intervening sequence. Ogden, R.C., Beckman, J.S., Abelson, J., Kang, H.S., Söll, D., Schmidt, O. Cell (1979) [Pubmed]
  25. Somatic expression of herpes thymidine kinase in mice following injection of a fusion gene into eggs. Brinster, R.L., Chen, H.Y., Trumbauer, M., Senear, A.W., Warren, R., Palmiter, R.D. Cell (1981) [Pubmed]
  26. Transgenic mice harboring SV40 T-antigen genes develop characteristic brain tumors. Brinster, R.L., Chen, H.Y., Messing, A., van Dyke, T., Levine, A.J., Palmiter, R.D. Cell (1984) [Pubmed]
  27. A novel type of transposon generated by insertion element IS102 present in a pSC101 derivative. Machida, Y., Machida, C., Ohtsubo, E. Cell (1982) [Pubmed]
  28. Two hybrid plasmids with D. melanogaster DNA sequences complementary to mRNA coding for the major heat shock protein. Schedl, P., Artavanis-Tsakonas, S., Steward, R., Gehring, W.J., Mirault, M.E., Goldschmidt-Clermont, M., Moran, L., Tissières, A. Cell (1978) [Pubmed]
  29. Fusion of the Escherichia coli tRNALeu1 promoter to the galK gene: analysis of sequences necessary for growth-rate-dependent regulation. Duester, G., Elford, R.M., Holmes, W.M. Cell (1982) [Pubmed]
  30. Genetic analysis of T-DNA transcripts in nopaline crown galls. Joos, H., Inzé, D., Caplan, A., Sormann, M., Van Montagu, M., Schell, J. Cell (1983) [Pubmed]
  31. A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription. Newport, J., Kirschner, M. Cell (1982) [Pubmed]
  32. Supercoiling of intracellular DNA can occur in eukaryotic cells. Giaever, G.N., Wang, J.C. Cell (1988) [Pubmed]
  33. The carboxy-terminal 30 amino acids of GAL4 are recognized by GAL80. Ma, J., Ptashne, M. Cell (1987) [Pubmed]
  34. RNA-mediated recombination in S. cerevisiae. Derr, L.K., Strathern, J.N., Garfinkel, D.J. Cell (1991) [Pubmed]
  35. Lariat structures are in vivo intermediates in yeast pre-mRNA splicing. Domdey, H., Apostol, B., Lin, R.J., Newman, A., Brody, E., Abelson, J. Cell (1984) [Pubmed]
  36. Genetic recombination of homologous plasmids catalyzed by cell-free extracts of Saccharomyces cerevisiae. Symington, L.S., Fogarty, L.M., Kolodner, R. Cell (1983) [Pubmed]
  37. Immunoprophylaxis of allergen-induced immunoglobulin E synthesis and airway hyperresponsiveness in vivo by genetic immunization. Hsu, C.H., Chua, K.Y., Tao, M.H., Lai, Y.L., Wu, H.D., Huang, S.K., Hsieh, K.H. Nat. Med. (1996) [Pubmed]
  38. Enzymatic replication of E. coli chromosomal origin is bidirectional. Kaguni, J.M., Fuller, R.S., Kornberg, A. Nature (1982) [Pubmed]
  39. Restoration of excitation-contraction coupling and slow calcium current in dysgenic muscle by dihydropyridine receptor complementary DNA. Tanabe, T., Beam, K.G., Powell, J.A., Numa, S. Nature (1988) [Pubmed]
  40. An unusual DNA structure detected in a telomeric sequence under superhelical stress and at low pH. Lyamichev, V.I., Mirkin, S.M., Danilevskaya, O.N., Voloshin, O.N., Balatskaya, S.V., Dobrynin, V.N., Filippov, S.A., Frank-Kamenetskii, M.D. Nature (1989) [Pubmed]
  41. A surface protease and the invasive character of plague. Sodeinde, O.A., Subrahmanyam, Y.V., Stark, K., Quan, T., Bao, Y., Goguen, J.D. Science (1992) [Pubmed]
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