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Gene Review:

pfoR - regulatory protein

Clostridium perfringens str. 13

Awad, M.M. et al., Shimizu, T. et al., Savic, D.J. et al.

Savic, McShan, Ferretti,

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Disease relevance of pfoR

Finally, we demonstrated that sloR, a S. pyogenes gene that closely resembles the Clostridium perfringens pfoR gene, exerts a negative effect on the expression of the slo gene [1].
Previous studies (T. Shimizu, A. Okabe, J. Minami, and H. Hayashi, Infect. Immun. 59:137-142, 1991) carried out with Escherichia coli suggested that the perfringolysin O structural gene, pfoA, was positively regulated by the product of the upstream pfoR gene [2].

High impact information on pfoR

These results indicate that pfoR positively controls expression of the pfoA gene [3].
The deduced amino acid sequence of pfoR possesses several motifs that are characteristic of DNA-binding proteins [3].
Nucleotide sequencing showed that this region contained one open reading frame (pfoR) coding for 343 amino acids [3].
In an attempt to confirm this hypothesis in C. perfringens, a pfoR-pfoA deletion mutant was complemented with isogenic pfoA(+) shuttle plasmids that varied only in their ability to encode an intact pfoR gene [2].

References

Autonomous expression of the slo gene of the bicistronic nga-slo operon of Streptococcus pyogenes. Savic, D.J., McShan, W.M., Ferretti, J.J. Infect. Immun. (2002) [Pubmed]
Perfringolysin O expression in Clostridium perfringens is independent of the upstream pfoR gene. Awad, M.M., Rood, J.I. J. Bacteriol. (2002) [Pubmed]
An upstream regulatory sequence stimulates expression of the perfringolysin O gene of Clostridium perfringens. Shimizu, T., Okabe, A., Minami, J., Hayashi, H. Infect. Immun. (1991) [Pubmed]

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