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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Chromosome Walking

 
 
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High impact information on Chromosome Walking

  • We have now identified PSF in the MHC class II region by deletion mapping in mutants and chromosome-walking [1].
  • To investigate how caspase-12 is transcriptionally and translationally regulated, we isolated and sequenced the 5'-flanking region of mouse caspase-12 gene by a PCR-mediated chromosome-walking technique, using mouse genomic DNA as a template [2].
  • The topoisomerase II clone was used as the starting point for chromosome-walking to isolate MKT1 [3].

References

  1. A gene in the human major histocompatibility complex class II region controlling the class I antigen presentation pathway. Spies, T., Bresnahan, M., Bahram, S., Arnold, D., Blanck, G., Mellins, E., Pious, D., DeMars, R. Nature (1990) [Pubmed]
  2. Molecular cloning and characterization of murine caspase-12 gene promoter. Oubrahim, H., Wang, J., Stadtman, E.R., Chock, P.B. Proc. Natl. Acad. Sci. U.S.A. (2005) [Pubmed]
  3. Molecular characterization of chromosomal genes affecting double-stranded RNA replication in Saccharomyces cerevisiae. Icho, T., Lee, H.S., Sommer, S.S., Wickner, R.B. Basic Life Sci. (1986) [Pubmed]
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