Disease relevance of MKT1

• MKT1 is required for maintenance of K2 above 30 degrees C in strains with the L-A-HN variant of the L-A double-stranded RNA virus of Saccharomyces cerevisiae [1].

High impact information on MKT1

• To address this issue directly, we mapped three quantitative trait loci that control yeast sporulation efficiency to single-nucleotide resolution in a noncoding regulatory region (RME1) and to two missense mutations (TAO3 and MKT1) [2].
• [NEX] replication depends on MAK3 but is independent of MAK4, MAK6, MAK27 and MKT1 [3].
• Posttranscriptional regulation of HO expression by the Mkt1-Pbp1 complex [4].
• Loss of MKT1 had little effect on HO mRNA levels but resulted in decreased protein levels [4].
• I mapped MKT1 near TOP2 and isolated the gene by chromosome walking from TOP2 [5].

Biological context of MKT1

• The recessive natural-variant mutations are not deletions as judged by Southern blots, but deletions of the MKT1 gene constructed in vitro and used to replace the normal gene surprisingly resulted in the same phenotype as that of the mkt1 natural variants, namely, a temperature-dependent maintenance of M2 double-stranded RNA [5].
• MKT1 is dispensable for host cell growth, mating, meiosis, and spore germination [5].

Other interactions of MKT1

• We cloned MAK11, MAK18, and MKT1 utilizing their genetic map positions [6].
• In this work, we analyzed three Saccharomyces cerevisiae high-temperature growth (Htg) QTGs (MKT1, END3, and RHO2) [7].
• Interestingly, while the MKT1 and END3 coding polymorphisms contribute to phenotype, it is the RHO2 3'UTR polymorphisms that are phenotypically relevant [7].
• We report that MKT1 encodes a 92,979 Da protein with serine-rich regions and the retroviral protease signature, DTG, but with no substantial homology to proteins presently in the databases [1].

Analytical, diagnostic and therapeutic context of MKT1

• The topoisomerase II clone was used as the starting point for chromosome-walking to isolate MKT1 [6].

References