Retroviral vectors preloaded with a viral receptor-ligand bridge protein are targeted to specific cell types.
Successful targeting methods represent a major hurdle to the use of retroviral vectors in cell-specific gene-delivery applications. We recently described an approach for retroviral targeting with a retroviral receptor-ligand bridge protein that was bound to the cognate cell-surface ligand receptors before viral challenge. We now report a significant improvement made to this viral targeting method by using a related bridge protein, designated TVB- EGF, comprised of the extracellular domain of the TVB receptor for subgroup B avian leukosis virus fused to epidermal growth factor ( EGF). The most important activity of TVB- EGF was that it allowed specific viral entry when preloaded onto virions. Furthermore, virions preloaded with TVB- EGF were thermostable and could be produced directly from virus- packaging cells. These data suggest an approach for targeting retroviral vectors to specific cell types by using virions preloaded with a retroviral receptor-ligand bridge protein and indicate that these types of bridge proteins may be useful reagents for studying the normal mechanism of retroviral entry.[1]References
- Retroviral vectors preloaded with a viral receptor-ligand bridge protein are targeted to specific cell types. Boerger, A.L., Snitkovsky, S., Young, J.A. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
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