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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Identification of Enterobacteriaceae by partial sequencing of the gene encoding translation initiation factor 2.

Nucleotide sequence analysis is increasingly being used to identify bacteria. In this work, a PCR assay based on degenerate primers was used to obtain the partial sequence of infB, the gene encoding translation initiation factor 2 ( IF2), in 39 clinical isolates of different Enterobacteriaceae. The partial sequence encodes the GTP-binding domain of IF2. Together with sequences from the literature, a total of 15 species, each represented by one to seven strains, was investigated. Phylogenetic analysis yielded an evolutionary tree which had a topology similar to a tree constructed using available 16S rRNA sequences. It is concluded that the inter-species variation of the infB gene fragment is sufficient for its use in the characterization of strains that have aberrant phenotypic reactions.[1]

References

  1. Identification of Enterobacteriaceae by partial sequencing of the gene encoding translation initiation factor 2. Hedegaard, J., Steffensen, S.A., Nørskov-Lauritsen, N., Mortensen, K.K., Sperling-Petersen, H.U. Int. J. Syst. Bacteriol. (1999) [Pubmed]
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