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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

A high-performance liquid chromatographic method for the determination of monofluoroacetate.

A simple isocratic high-performance liquid chromatographic (HPLC) method for the quantitative analysis of monofluoroacetic acid (MFA), the toxic substance of Dichapetalum cymosum, in plant material, rumen contents (gastric contents), and liver samples is described. A suitable HPLC column that gives optimum sensitivity, accuracy, precision, and separation of MFA is identified. A C-610 organic acid analysis column at ambient temperature with 0.02M H3PO4 as an eluent and ultraviolet detection at 210 nm is utilized to quantitate MFA. Using this method, the average percentage recovery in plant material, bovine liver, and rumen samples is 94.8%, and a detection limit of 12 microg/L is achievable.[1]


  1. A high-performance liquid chromatographic method for the determination of monofluoroacetate. Minnaar, P.P., Swan, G.E., McCrindle, R.I., de Beer, W.H., Naudé, T.W. Journal of chromatographic science. (2000) [Pubmed]
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