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Vera-Avila, L.E. et al.

Trace-level determination of benzidine and 3,3'-dichlorobenzidine in aqueous environmental samples by online solid-phase extraction and liquid chromatography with electrochemical detection.

A simple, rapid, and reliable online methodology for the determination of benzidine and 3,3'-dichlorobenzidine (3,3'-DCB) in natural waters is proposed. The analytes are extracted and preconcentrated from aqueous samples in a small stainless steel precolumn packed with a polymeric PLRP-S phase. The precolumn is further online-analyzed by reversed-phase gradient-elution chromatography with a highly sensitive and selective coulometric detection at E = 700 mV. Recoveries greater than 90% and a relative standard deviation of approximately 5% are achieved with samples spiked at low micrograms-per-liter concentration levels. The detection limits of the method in fortified reagent water samples are 100 ng/L for benzidine and 50 ng/L for 3,3'-DCB.[1]

References

Trace-level determination of benzidine and 3,3'-dichlorobenzidine in aqueous environmental samples by online solid-phase extraction and liquid chromatography with electrochemical detection. Vera-Avila, L.E., García-Ac, A., Covarrubias-Herrera, R. Journal of chromatographic science. (2001) [Pubmed]

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