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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Structural determination of the subsidiary colors in food blue No. 1 (brilliant blue FCF) aluminum lake detected by paper chromatography.

One of eight Food Blue No. 1 aluminum lakes (B-1Als) used in the official inspection of coal-tar colors in fiscal year 1999 had a violet sub-spot during paper chromatography and was rejected. To clarify the orgin of the sub-spot, the violet subsidiary color (Sub-V) was isolated from the sample. On the basis of NMR and MS analyses and ion chromatography, the structure of the subsidiary color was elucidated to be 2-[[4-[N-ethyl-N-(3- sulfophenylmethyl)amino]phenyl][4-hydroxyphenyl]methylio]benzenesulfonic acid. The relative content of Sub-V to that of m,m-B-1 in the rejected sample was determined to be 39.5% by HPLC. The relative contents in other submitted samples of B-1Al were in the range of 1.1-3.6%.[1]

References

  1. Structural determination of the subsidiary colors in food blue No. 1 (brilliant blue FCF) aluminum lake detected by paper chromatography. Tsuji, S., Amakura, Y., Umino, Y., Nishi, M., Nakanishi, T., Tonogai, Y. Shokuhin Eiseigaku Zasshi (2001) [Pubmed]
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