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Sac I Restriction Fragment Length Polymorphism (RFLP) related to the human CST2 gene.

Restriction Fragment Length Polymorphism (RFLP) related to cystatin gene (CST) family was detected in the Japanese population by using restriction enzyme Sac I. A polymorphic site, located at 0.9 kb from the 3' end of the CST2 gene, revealed a two allele polymorphism with band sizes of 3.5 kb and 8.3 kb by hybridization with probe including exon 2 of the CST1 gene. The gene frequencies in the Japanese population were 0.326 for 3.5 kb allele and 0.674 for 8.3 kb allele (n = 86). The phenotypes of the polymorphism showed no association with the previously reported electrophoretic cystatin SA protein phenotypes.[1]


  1. Sac I Restriction Fragment Length Polymorphism (RFLP) related to the human CST2 gene. Minaguchi, K., Kiriyama, T., Saitoh, E., Isemura, S., Sanada, K. Bull. Tokyo Dent. Coll. (2002) [Pubmed]
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