A method for the analysis of low-mass molecules by MALDI-TOF mass spectrometry.
We report a novel method termed matrix suppressed laser desorption/ionization to improve the analysis of low-mass molecules by MALDI-TOF mass spectrometry. In this method, the surfactant of cetrimonium bromide (CTAB) is added to the conventional matrix of alpha-cyano-4-hydroxycinnamic acid solution to prepare the MALDI samples. During the MALDI process, the presence of CTAB could substantially or even completely suppress the matrix-related ion background. As a result, very clean mass spectra can be routinely obtained in the low-mass range. In addition, the presence of CTAB can significantly improve the mass resolution of low-mass molecules. It is seen that high-quality spectra were routinely obtained at a matrix/CTAB ratio of 1000:1. This method has been successfully used to analyze a variety of low-mass molecules.[1]References
- A method for the analysis of low-mass molecules by MALDI-TOF mass spectrometry. Guo, Z., Zhang, Q., Zou, H., Guo, B., Ni, J. Anal. Chem. (2002) [Pubmed]
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