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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Simultaneous detection of hippuric acid and methylhippuric acid in urine by Empore disk and gas chromatography-mass spectrometry.

A method is described for the determination of hippuric acid (HA) and o-, m-, and p-methylhippuric acids (o-, m-, p-MHAs) in urine using solid-phase extraction and gas chromatography-mass spectrometry (GC-MS). The extraction procedure uses an Empore disk, derivatized into the respective trimethyl silyl derivatives. All metabolites including the internal standard (I.S.) were clearly able to be analyzed by the DB-17 column. The calibration curves for the four acids show linearity in the range of 5-70 microg/ml. The detection limit of each acid was 1.0-2.5 microg/ml.[1]

References

  1. Simultaneous detection of hippuric acid and methylhippuric acid in urine by Empore disk and gas chromatography-mass spectrometry. Saito, T., Takeichi, S. Journal of pharmaceutical and biomedical analysis. (2002) [Pubmed]
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