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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Reactions of cellulose isothiocyanates with thiol and amino compounds.

A cellulose isothiocyanate has been prepared by treatment of cellulose with 2,4-di-isocyanatotoluene followed by hydrolysis and reaction of the resulting amine with thiophosgene. The cellulose isothiocyanate was characterized by its binding capacity with respect to [14C]-glycine, [131 I]-human serum albumin, and 2-mercaptoethanol. An analytical method for binding capacity, based on reaction with [35 S]-alpha-toluenethiol, was developed. Because of the aromatic character of the NCS group of the cellulose isothiocyanate, the covalently bonded thiol can be quantitatively liberated.[1]


  1. Reactions of cellulose isothiocyanates with thiol and amino compounds. Gemeiner, P., Augustín, J., Drobnica, L. Carbohydr. Res. (1977) [Pubmed]
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