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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Electrospray-assisted laser desorption/ionization mass spectrometry for direct ambient analysis of solids.

A new method of electrospray-assisted laser desorption/ionization (ELDI) mass spectrometry, which combines laser desorption with post-ionization by electrospray, was applied to rapid analysis of solid materials under ambient conditions. Analytes were desorbed from solid metallic and insulating substrata using a pulsed nitrogen laser. Post-ionization produced high-quality mass spectra characteristic of electrospray, including protein multiple charging. For the first time, mass spectra of intact proteins were obtained using laser desorption without adding a matrix. Bovine cytochrome c and an illicit drug containing methaqualone were chosen in this study to demonstrate the applicability of ELDI to the analysis of proteins and synthetic organic compounds.[1]

References

  1. Electrospray-assisted laser desorption/ionization mass spectrometry for direct ambient analysis of solids. Shiea, J., Huang, M.Z., Hsu, H.J., Lee, C.Y., Yuan, C.H., Beech, I., Sunner, J. Rapid Commun. Mass Spectrom. (2005) [Pubmed]
 
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