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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Improved multiplex PCR method for the rapid detection of beta-lactamase genes in Escherichia coli of animal origin.

We developed 2 variants, A and B, of a multiplex polymerase chain reaction method for detecting the beta-lactam resistance genes bla(TEM), bla(SHV), and bla(OXA) in 122 uropathogenic Escherichia coli animal strains. Method B yielded 98% specificity and 100% sensitivity, and method A yielded 100% and 89%, respectively. Variant B was more accurate (99%) than A (94%).[1]

References

  1. Improved multiplex PCR method for the rapid detection of beta-lactamase genes in Escherichia coli of animal origin. Pomba, C., Mendonça, N., Costa, M., Louro, D., Baptista, B., Ferreira, M., Correia, J.D., Caniça, M. Diagn. Microbiol. Infect. Dis. (2006) [Pubmed]
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