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Prediction of measurement uncertainty in isotope dilution gas chromatography/ mass spectrometry.

An equation is theoretically derived which describes the relative standard deviation (RSD) of the amount ratios of analyte to its isotope-labeled variant in gas chromatography/ mass spectrometry (GC/MS) using the stable isotope dilution method. The determination of methyltestosterone is taken as an example. The uncertainty equation proposed is justified by comparing the theoretical RSD values with the experimental RSD values obtained by replication over a wide range of analyte amount. The detection limit and quantitation limit are estimated from the continuous plot (precision profile) of the theoretical RSD against analyte amount.[1]

References

  1. Prediction of measurement uncertainty in isotope dilution gas chromatography/mass spectrometry. Hasegawa, H., Shinohara, Y., Hashimoto, T., Matsuda, R., Hayashi, Y. Journal of chromatography. A (2006) [Pubmed]
 
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