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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Determination of selenium(IV) in pharmaceuticals and premixes by micellar liquid chromatography.

A simple micellar liquid chromatographic technique for the determination of selenium(IV) in pharmaceutical products (multi-vitamin tablets, syrups) and animal premixes after pre-column derivatization with 2,3-diaminonaphthalene was developed and validated. Hypersil ODS column, 10% (v/v) 1-butanol in 0.05 M sodium dodecyl sulfate as the mobile phase and UV detection at 378 nm and were used. The retention time was about 8 min. In the course of the validation study, the specificity of the method was demonstrated. Linearity was established in the range 0.33-3.3 microg ml(-1) of selenium(IV) content. The limits of detection and quantitation were 0.1 and 0.3 microg ml(-1), respectively. The method showed excellent accuracy (100.04%). Precision (repeatability) gave a relative standard deviation less than 1%. The influence of the various method parameters (robustness study) was also investigated.[1]


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