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Caws, M. et al.

Maxine Caws, Dau Quang Tho, Phan Minh Duy, Nguyen Thi Ngoc Lan, Dai Viet Hoa, Mili Estee Torok, Tran Thi Hong Chau, Nguyen Van Vinh Chau, Nguyen Tran Chinh, Jeremy Farrar,

PCR-restriction fragment length polymorphism for rapid, low-cost identification of isoniazid-resistant Mycobacterium tuberculosis.

PCR-restriction fragment length poymorphism (PCR-RFLP) is a simple, robust technique for the rapid identification of isoniazid-resistant Mycobacterium tuberculosis. One hundred consecutive isolates from a Vietnamese tuberculosis hospital were tested by MspA1I PCR-RFLP for the detection of isoniazid-resistant katG_315 mutants. The test had a sensitivity of 80% and a specificity of 100% against conventional phenotypic drug susceptibility testing. The positive and negative predictive values were 1 and 0.86, respectively. None of the discrepant isolates had mutant katG_315 codons by sequencing. The test is cheap (less than $1.50 per test), specific, and suitable for the rapid identification of isoniazid resistance in regions with a high prevalence of katG_315 mutants among isoniazid-resistant M. tuberculosis isolates.[1]

References

PCR-restriction fragment length polymorphism for rapid, low-cost identification of isoniazid-resistant Mycobacterium tuberculosis. Caws, M., Tho, D.Q., Duy, P.M., Lan, N.T., Hoa, D.V., Torok, M.E., Chau, T.T., Chau, N.V., Chinh, N.T., Farrar, J. J. Clin. Microbiol. (2007) [Pubmed]

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