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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

A new purification procedure for bovine C-reactive protein and serum amyloid P component.

1. A new purification procedure was started with salting-out fractionation of serum proteins at 45-75% saturated ammonium sulfate concentration, followed by HE agarose affinity chromatography by which calcium-dependently bound CRP and SAP were purely eluted with EDTA-containing buffer. 2. Pure CRP and SAP were finally separated by DEAE-5PW HPLC. 3. This procedure gave recovery of 15 and 26%, and fold purification of 2650 and 2400 for CRP and SAP, respectively. 4. Each subunit of CRP and SAP had one intrasubunit disulfide bond, determined by reduction and carboxymethylation.[1]

References

  1. A new purification procedure for bovine C-reactive protein and serum amyloid P component. Sarikaputi, M., Morimatsu, M., Syuto, B., Saito, M., Naiki, M. Int. J. Biochem. (1991) [Pubmed]
 
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