Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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van Ginneken, V. et al.

V. van Ginneken, E. Verhey, R. Poelmann, R. Ramakers, K. Willems van Dijk, Lisanne Ham, P. Voshol, L. Havekes, M. Van Eck, J. van der Greef,

Metabolomics (liver and blood profiling) in a mouse model in response to fasting: a study of hepatic steatosis.

A metabolomic approach was applied to a mouse model of starvation-induced hepatic steatosis. After 24 h of fasting it appears that starvation reduced the phospholipids (PL), free cholesterol (FC), and cholesterol esters (CE) content of low-density lipoproteins (LDL). In liver lipid profiles major changes were observed using different techniques. High performance thin layer chromatography (HPTLC)-measurements of liver-homogenates indicated a significant rise of FC with 192%, triacylglycerols (TG) with 456% and cholesterol esters (CE) with 268% after 24 h of starvation in comparison with the control group. Reversed phase liquid chromatography coupled to mass spectrometry measurements (LC-MS) of liver homogenate indicated that the intensity of Phosphatidylcholine (PC) in the 24-h starvation group dropped to 90% of the value in the control group while the intensity of CE and TG increased to 157% and 331%, respectively, of the control group. Interestingly, a 49:4-TG with an odd number of C atoms appeared during starvation. This unique triacylglycerol has all characteristics of a biomarker for detection of hepatic steatosis. These observations indicate that in mammals liver lipid profiles are a dynamic system which are readily modulated by environmental factors like starvation.[1]

References

Metabolomics (liver and blood profiling) in a mouse model in response to fasting: a study of hepatic steatosis. van Ginneken, V., Verhey, E., Poelmann, R., Ramakers, R., van Dijk, K.W., Ham, L., Voshol, P., Havekes, L., Van Eck, M., van der Greef, J. Biochim. Biophys. Acta (2007) [Pubmed]

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