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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Bovine galactosyltransferase: identification of a clone by direct immunological screening of a cDNA expression library.

A 1.3-kilobase cDNA clone (7A) coding for bovine galactosyltransferase (glycoprotein 4-beta-galactosyltransferase, EC 2.4.1.38) was isolated from a lambda gt11 expression library by immunological screening with monospecific polyclonal antisera to the affinity-purified bovine enzyme. The nucleotide sequence of this clone predicts an open reading frame that starts at the 5' end of the insert and codes for a polypeptide of 334 amino acids with Mr 37,645. Based on a Mr of 57,000 for the membrane-bound enzyme this clone accounts for approximately 61% of the coding sequence. Portions of the predicted amino acid sequence matched the six tryptic peptides isolated from affinity-purified bovine galactosyltransferase. Clone 7A hybridizes to a 4.8-kilobase bovine mRNA and identifies multiple EcoRI restriction fragments in bovine, murine, and human DNA.[1]

References

  1. Bovine galactosyltransferase: identification of a clone by direct immunological screening of a cDNA expression library. Shaper, N.L., Shaper, J.H., Meuth, J.L., Fox, J.L., Chang, H., Kirsch, I.R., Hollis, G.F. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
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