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Eckstein, F. et al.

Phosphorothioates in molecular biology.

The observation that phosphorothioate analogues of the nucleoside triphosphates are substrates for DNA- and RNA-polymerases has proven a boon for the molecular biologist. As these phosphorothioate-containing polymers are stable to degradation by nucleases and the sulfur atom confers many favourable chemical properties, several applications in molecular biology have been developed, including new methods for site-directed mutagenesis and DNA sequencing.[1]

References

Phosphorothioates in molecular biology. Eckstein, F., Gish, G. Trends Biochem. Sci. (1989) [Pubmed]

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