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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Isolation of a cDNA for the rat heavy neurofilament polypeptide (NF-H).

We have isolated from a rat brain lambda gt11 expression library two overlapping cDNA clones of sizes 2.5 and 3.0 kb corresponding to the heavy neurofilament polypeptide (NF-H). The 2.5 kb insert apparently represents virtually the whole of the C-terminal tail, the 3.0 kb insert also encodes the conserved epitope for the monoclonal antibody, anti-IFA. The identity of the cDNAs was established by comparison of the predicted amino acid sequence with the known partial amino acid sequence of porcine NF-H. A repeat peptide sequence that may be a multiphosphorylation site was identified in the C-terminal non-helical tail.[1]


  1. Isolation of a cDNA for the rat heavy neurofilament polypeptide (NF-H). Robinson, P.A., Wion, D., Anderton, B.H. FEBS Lett. (1986) [Pubmed]
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