Sequencing and heterologous expression of the gene encoding penicillin V amidase from Bacillus sphaericus.
The Bacillus sphaericus gene encoding penicillin V amidase, which catalyzes the hydrolysis of penicillin V, has been characterized. The entire nucleotide sequence of the coding region, as well as 5'- and 3'-flanking regions, was determined using an improved sequencing strategy. The deduced amino acid sequence suggests a protein consisting of 338 residues with an Mr of 37,500. The ATG initiator codon is preceded by a putative ribosome-binding site, typical for genes of Gram-positive origin. High expression of the gene was obtained in Escherichia coli using an inducible promoter, showing that the gene product is stable in this heterologous host.[1]References
- Sequencing and heterologous expression of the gene encoding penicillin V amidase from Bacillus sphaericus. Olsson, A., Uhlén, M. Gene (1986) [Pubmed]
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