Kinetics of recB-dependent repair: relationship to post-UV inactivation of the prophage.
By making use of the temperature-sensitive mutant recB270, we showed that the RecBCD enzyme is needed for repair between 1 and 4 h after UV exposure. recB-dependent prophage inactivation (Petranović et al. (1984), Mol. Gen. Genet., 196, 167-169) takes place in all dying cells during the same period of time. The kinetics of decrease in the yield of recombinants in phage-propage crosses resemble those of prophage inactivation in UV-irradiated bacteria. This indicates that recombination processes (including site-specific recombination required for prophage excision) are blocked in cells destined to die. On the basis of our results, we suggest that a large fraction of damaged cells is rescued by the RecA-RecBCD recombination pathway. If repair is unsuccessful, RecA-RecBCD recombination intermediates persist in the irradiated cells leading to prophage inactivation.[1]References
- Kinetics of recB-dependent repair: relationship to post-UV inactivation of the prophage. Trgovcević, Z., Petranović, D., Salaj-Smic, E., Petranović, M. Mutat. Res. (1987) [Pubmed]
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