A convenient radiometric assay for flavin-containing monooxygenase activity.
A rapid, convenient assay to determine the activity of the flavin-containing monooxygenase is described. The method is based on direct analysis of quenched incubation mixtures by thin-layer chromatography and utilizes tritiated dimethylaniline as the substrate. The synthesis of the radiolabeled substrate is described. The usefulness of dimethylaniline N-oxide formation as a measure of flavin-containing monooxygenase activity was assessed using the purified hog liver enzyme, hog liver microsomes, and liver microsomes from untreated and phenobarbital-pretreated rats.[1]References
- A convenient radiometric assay for flavin-containing monooxygenase activity. Cashman, J.R. Anal. Biochem. (1987) [Pubmed]
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