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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

An improved method for preparation of C1-depleted serum and its application to hemolytic assay of C1.

Serum depleted of the first component of complement ( C1D) was prepared by treating fresh human serum with Sepharose-IgG in the presence of triethylenetetramine-N,N,N',N'',N''',N'''-hexa-acetic acid and di-isopropyl fluorophosphate at acidic pH (5.2). The total hemolytic activity of this C1D could be increased to approximately 75% of that of the original serum by the addition of excess purified C1. A linear relation was obtained on determination of C1 hemolytic activity using C1D, and the C1 titers of sera from patients measured by this simple method showed a good correlation with those measured using intermediate cells.[1]

References

  1. An improved method for preparation of C1-depleted serum and its application to hemolytic assay of C1. Fukumoto, Y., Tsumoto, K., Tashiro, A., Ohtomo, N., Yoshida, T. Complement (1985) [Pubmed]
 
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