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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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On the synthesis of a 32P-labelled Edman reagent for the sensitive identification of amino-acid derivatives.

A phosphorus-32 containing derivative of phenylisothiocyanate was prepared to increase the sensitivity of amino-acid sequence determination. The respective compound 2-(4-isothiocyanatophenoxy)-1,3,2-dioxaphosphinane 2-oxide showed about the same reactivity, stability, and polarity as the Edman reagent itself. A repetitive yield of 94% was obtained in the stepwise degradation of insulin B chain using a solid phase sequencer. The synthesis of this radioactive reagent was achieved within 5 h but with a specific activity of 1 Ci/ mol. Eight amino acids were reacted with the 32P-labelled reagent and identified by autoradiography after two dimensional thin-layer chromatography.[1]

References

  1. On the synthesis of a 32P-labelled Edman reagent for the sensitive identification of amino-acid derivatives. Ender, B.E., Gassen, H.G., Machleidt, W. Hoppe-Seyler's Z. Physiol. Chem. (1984) [Pubmed]
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