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Hybridization and isolation of human-rat beta gamma enolase.

A human-rat hybrid form of enolase (beta gamma) was prepared by dissociation and reassociation of a mixture of human beta beta and rat gamma gamma enolases, followed by isolation of the hybrid form from the parental homodimeric enolases with DEAE-Sephadex column chromatography. The human-rat beta gamma enolase had a specific activity similar to those of human beta beta and rat gamma gamma enolases. The optimal pH, stability against heating, and Km for 2-phosphoglycerate of the hybrid enolase were also similar to those of the homodimeric enolases.[1]

References

  1. Hybridization and isolation of human-rat beta gamma enolase. Shimizu, A., Kato, K. Biochim. Biophys. Acta (1984) [Pubmed]
 
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