The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Further improvement of dye-exclusion microcytotoxicity assay by introducing low melting point agarose into the medium.

We developed an improved method for the dye-exclusion microcytotoxicity test by introducing low melting point agarose ( LMP- Aga) into the assay medium. The procedures in our method are made very feasible by such noble characteristics of LMP- Aga that once solubilized, the sol state is retained at 37 degrees C, while once gelated, the gelated state is retained even at 37 degrees C. The most important advantage of our method is that even distribution, without any accumulation, of the target cells on the bottom surface of the test wells and no dislodgement of the target cells from the wells by mechanical force inflicted during the assay, enable us to observe the target cells precisely and to obtain an accurate percentage of dead target cells. Another advantage of the present method is that in complement-mediated cytotoxicity tests, it is possible to use unabsorbed normal serum as complement source because naturally occurring cytotoxicity in normal serum is completely removed during the diffusion into the LMP- Aga medium. Thus it should be concluded that our method is excellent in all respects, e.g., accuracy and reproducibility in the results of the test, and feasibility of the procedures.[1]

References

 
WikiGenes - Universities