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Cesarone, C.F. et al.

DNA damage induced in vivo in various tissues by nitrochlorobenzene derivatives.

Mono-, di-, and trinitrochlorobenzenes were injected i.p. into albino Swiss CD1 mice. Their effects were evaluated, in brain, liver and kidney, as single-strand DNA breaks. DNA damage was recognizable 4 h after administration in vivo, and its increment seemed to be related to the number of nitro groups contained in the chlorobenzene molecule. The simple and accurate microfluorometric procedure for DNA assay associated to the alkaline elution technique improved the application in vivo, avoiding the radiolabeling of DNA.[1]

References

DNA damage induced in vivo in various tissues by nitrochlorobenzene derivatives. Cesarone, C.F., Bolognesi, C., Santi, L. Mutat. Res. (1983) [Pubmed]

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