Purification of His-Tag fusion proteins from Escherichia coli.
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses a contaminating protein from Escherichia coli found when histidine-tagged fusion proteins are purified by metal chelate affinity chromatography. For details on how to partake in the newsgroup, see the accompanying box.[1]References
- Purification of His-Tag fusion proteins from Escherichia coli. Hengen, P. Trends Biochem. Sci. (1995) [Pubmed]
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