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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Simple and sensitive quantitation method for mevalonic acid in plasma using gas chromatography/ mass spectrometry.

The combination of gas chromatography and mass spectrometry using a polar capillary column and isobutane chemical ionization made it possible to determine mevalonic acid (MVA) as the lactone at subnanogram levels using 1 mL of plasma. The pretreatment procedure consisted of only three steps, namely lactonization, washing with chloroform and liquid/liquid extraction. This simple, rapid and sensitive method, having good precision and accuracy, is useful for evaluating the change of plasma MVA, which is well correlated with whole-body cholesterol biosynthesis.[1]

References

  1. Simple and sensitive quantitation method for mevalonic acid in plasma using gas chromatography/mass spectrometry. Ishihama, Y., Mano, N., Oda, Y., Hiyoshi, H., Ikuta, H., Yoshida, Y. Rapid Commun. Mass Spectrom. (1994) [Pubmed]
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