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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Modification of tryptophan 149 of inorganic pyrophosphatase from Escherichia coli.

1. The inorganic pyrophosphatase from Escherichia coli was almost completely inactivated on chemical modification of Trp-149 with N-bromosuccinimide (NBS). 2. The presence of a complex of Mg2+ and a substrate analogue, iminodiphosphate ( PNP), provided considerable protection against the inactivation, whereas Mg2+ or PNP alone afforded only slight protection.[1]

References

  1. Modification of tryptophan 149 of inorganic pyrophosphatase from Escherichia coli. Kaneko, S., Ichiba, T., Hirano, N., Hachimori, A. Int. J. Biochem. (1993) [Pubmed]
 
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