Nucleotide sequence analysis of the CDNA for rat DNA topoisomerase II.
CDNA clones encoding the rat DNA topoisomerase II were isolated from rat testis CDNA library using a DNA probe synthesized by two sequential nested PCRs. The nucleotide sequence of the entire coding region and its deduced 1526 amino acid sequence showed that 80% nucleotides and 89% amino acids were identical with human HeLa DNA topoisomerase II gene (hTOP2). Approximately 1100 amino acids at the N-terminus shows 96.5% sequence identity, but C-terminus has only 65% homology. Rat DNA topoisomerase II gene (rTOP2) contains three functional domains responsible for ATPase activity, break-reunion activity, and complex stability and DNA binding activity like other eukaryotic TOP2. It also contains two putative nuclear targeting sequences and a leucine zipper motif and has highly charged species specific sequences at the C-terminus.[1]References
- Nucleotide sequence analysis of the CDNA for rat DNA topoisomerase II. Park, S.H., Yoon, J.H., Kwon, Y.D., Park, S.D. Biochem. Biophys. Res. Commun. (1993) [Pubmed]
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