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Cloning and nucleotide sequence of Bacillus stearothermophilus pyruvate carboxylase.

A gene for prokaryotic pyruvate carboxylase (PC) was cloned from Bacillus stearothermophilus. It has an open reading frame of 3441 base pairs which can code for a protein of 128,353 Da. Not only the molecular size and domain organization but also the deduced amino acid sequence of B. stearothermophilus PC are similar to those of eukaryotic PCs.[1]

References

  1. Cloning and nucleotide sequence of Bacillus stearothermophilus pyruvate carboxylase. Kondo, H., Kazuta, Y., Saito, A., Fuji, K. Gene (1997) [Pubmed]
 
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