A protein C pathway ( PCP) screening test for the detection of APC resistance and protein C or S deficiencies.
A new automated method for screening defects in the Protein C Pathway ( PCP) was evaluated. The " PCP test" is based on a phospholipid-rich Russells viper venom reagent, insensitive to heparin and lupus anticoagulants. To minimize interference from other clotting variables, ratios of the clotting time with and without the addition of a protein C activator were usually determined. Plasma samples from healthy volunteers, patients untreated or on oral anticoagulants, patients with factor V Leiden with and without treatment, and patients with protein C and/or S deficiencies were tested. Mixing patient plasmas 1:1 with individual plasmas deficient in factor V, protein C or S was evaluated for identifying the nature of defects by shortening the screening test. The PCP test was found to be sensitive to APC resistance due to factor V Leiden and by mixing with factor V deficient plasma was also useful despite the effects of oral anticoagulants. Results in the group of patients with previous low protein C or S levels suggest that the method has a better sensitivity to protein C than to protein S deficiency. The automated test was simple to use and gave a between-run coefficient of variation below 3% on normal plasmas.[1]References
- A protein C pathway (PCP) screening test for the detection of APC resistance and protein C or S deficiencies. Haas, F.J., van Sterkenburg-Kamp, B.M., Scheepers, H.A. Semin. Thromb. Hemost. (1998) [Pubmed]
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