Disease relevance of T2

• To investigate the kinetic changes in adaptive immunity during experimental Haemophilus influenzae type b (Hib) meningitis, we established a murine meningitis model based on T1/T2 doubly transgenic mice [1].

High impact information on T2

• Their distinct BCR-dependent responses suggest unique roles for T1 vs T2 cells in peripheral B cell selection [2].
• In addition, T2 cells from Bruton's tyrosine kinase-deficient Xid mice failed to generate these proliferative and survival responses, suggesting a requirement for the BCR signalosome specifically at the T2 stage [2].
• BCR stimulation of T2 cells also led to down-regulation of CD21 and CD24 (HSA) expression, resulting in a mature B cell phenotype [2].
• Taken together, these data clearly demonstrate that T2 immature B cells comprise a discrete developmental subset that mediates BCR-dependent proliferative, prosurvival, and differentiation signals [2].
• In response to BCR engagement, T2 cells rapidly entered cell cycle and resisted cell death [2].

Biological context of T2

• Sequence analysis of T2 from several t haplotypes shows that they all share the same changed stop codon, and, thus, T2 is a candidate gene for the t complex tail interaction factor [3].
• However, despite making a maternal connection, T2 homozygotes cease development at E11.5 and die soon after [4].
• The phenotype of T2 superficially resembles that of Brachyury; however, there are several important differences [4].

Anatomical context of T2

• A mutation in T2 disrupts notochord development, pointing to the existence of a second T/t complex gene involved in axis development [3].
• For Treatment 2 (T2), oocytes were matured in a portable incubator, in plastic tubes containing the same medium as T1 [5].
• T1, T2 and T3 resulted in 20.26, 16.94 and 24.43% development to morulae, and 4.01, 3.06 and 1.85% development to blastocysts, respectively (P >0.05) [5].

Other interactions of T2

• T1, T2, and the unlinked t-int are distinct and unrelated loci, and mutations in these genes do not complement one another genetically [3].
• Mouse Brachyury the Second (T2) is a gene next to classical T and a candidate gene for tct [3].

References