Disease relevance of CIZ1

• We have isolated and characterized another member of the matrin 3 family, designated NP94, from a medulloblastoma [1].
• Despite the emerging significance of Ciz1 in the biology of breast cancer cells, regulation of endogenous Ciz1 in hormone-responsive cancer cells remains unknown [2].
• In addition, we found that Ciz1 overexpression confers estrogen hypersensitivity to breast cancer cells and promotes the growth rate, anchorage independency, and tumorigenic properties of breast cancer cells [2].

High impact information on CIZ1

• These findings suggest that interactions among DLC1, Cdk2, and Ciz1 play a regulatory role in cell cycle progression of cancer cells presumably by influencing the levels of nuclear p21(WAF1) [3].
• Ciz1, a Novel DNA-Binding Coactivator of the Estrogen Receptor {alpha}, Confers Hypersensitivity to Estrogen Action [2].
• We also showed that down-regulation of DLC1 and Ciz1 reduced both Cdk2 activity and cell cycle progression of breast cancer ZR-75 and MCF-7 and endometrial Ishikawa cancer cells [3].
• C-terminal domains deliver the DNA replication factor Ciz1 to the nuclear matrix [4].
• This suggests that Ciz1 is normally immobilized by interaction with non-chromatin nuclear structures, consistent with the nuclear matrix [4].

Biological context of CIZ1

• Cloning and characterization of a novel p21(Cip1/Waf1)-interacting zinc finger protein, ciz1 [5].
• A region of about 150 amino acids containing the first zinc-finger motif in Ciz1 was the binding site for p21(Cip1/Waf1) [5].
• A modified selected and amplified binding (SAAB) sequence method was used to identify DNA sequences recognized by Ciz1 [1].
• We recently showed that Cip-interacting zinc finger protein 1 (Ciz1) participates in the regulation of the cell cycle in estrogen-stimulated breast cancer cells [2].

Associations of CIZ1 with chemical compounds

• Here, we report a novel p21(Cip1/Waf1)-interacting protein, Ciz1 (for Cip1 interacting zinc finger protein), which contains polyglutamine repeats and glutamine-rich region in the N-terminus as well as three zinc-finger motifs and one MH3 (matrin 3-homologous domain 3) in the C-terminal region [5].

Regulatory relationships of CIZ1

• Cip1-interacting zinc finger protein 1 (Ciz1) stimulates DNA replication in vitro and is required for mammalian cells to enter S phase [4].

Other interactions of CIZ1

• When Ciz1 and p21(Cip1/Waf1) were individually overexpressed in U2-OS cells, they mostly localized in the nucleus [5].

Analytical, diagnostic and therapeutic context of CIZ1

• From the analysis of the retrieved SAAB sequences and verification using electrophoretic mobility shift assays, we formulated a consensus DNA sequence, ARYSR(0-2)YYAC, recognized by Ciz1 [1].
• Analysis of green fluorescent protein-tagged fragments indicates that nuclear immobilization of Ciz1 is mediated by sequences in its C-terminal third, encoded within amino acids 708-830 [4].

References