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Gene Review

TAF15  -  TAF15 RNA polymerase II, TATA box binding...

Homo sapiens

Synonyms: 68 kDa TATA-binding protein-associated factor, Npl3, RBP56, RNA-binding protein 56, TAF(II)68, ...
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Disease relevance of TAF15


High impact information on TAF15

  • Moreover, we demonstrate that hTAF(II)68 co-purifies also with the human RNA polymerase II and can enter the preinitiation complex together with Pol II [3].
  • Here we report the cloning and characterization of a novel human TBP-associated factor, hTAF(II)68 [3].
  • Moreover, EWS is not exclusively associated with TFIID but, similarly to hTAFII68, is also associated with the Pol II complex [4].
  • Extraskeletal myxoid chondrosarcomas (EMCs) are characterized by recurrent t(9;22) or t(9;17) translocations resulting in fusions of the NH2-terminal transactivation domains of EWS or TAF2N to the entire TEC protein [5].
  • We demonstrate that the N-terminus of TAFII68 efficiently stimulates transcription when fused to two different DNA binding domains and that overexpression of TAFII68-FLI-1 chimeras in NIH3T3 cells leads to oncogenic transformation [6].

Biological context of TAF15

  • Investigating the multiple functions of TLS, EWS and TAF15 will enhance our understanding of gene expression as a whole, and also allow us to better understand how these proteins may be contributing to the oncogenic pathways the associated fusion proteins initiate [7].
  • We have determined that the translocation results in the fusion of the entire coding region of CHN to the N-terminal transactivation domain of RBP56/hTAFII68 [1].
  • The RBP56/hTAFII68 gene spans about 37kb and consists of 16 exons from 33bp to 562bp [8].
  • The RBP56 gene was mapped to chromosome 17q11.2 to q12 [9].
  • Taken together, our results suggest that the biological activities of hTAF(II)68 are linked to the cytoplasmic Src signal transduction pathway [10].

Associations of TAF15 with chemical compounds


Other interactions of TAF15

  • In the present study, we address the question whether EWS and hTAF(II)68 also display pairing on membrane activities, and to a larger extent whether other proteins also exhibit such activity [2].
  • One such multifunctional family is made up of the RNA-binding proteins TLS, EWS and TAF15 [7].
  • Molecular cloning of the translocations t(12;22)(p13;q12) and t(12;17)(p13;q11) in acute leukaemia showed that either EWSR1 or its homologue TAF15 are fused to the transcription factor CIZ [12].
  • The hTAF(II)68 protein can associated with the SH3 domains of several cell signaling proteins, including v-Src protein tyrosine kinase [10].
  • The subunits of Pol II that interact with EWS and hTAFII68 have been identified, confirming the association with the polymerase [4].

Analytical, diagnostic and therapeutic context of TAF15


  1. Identification of a novel fusion gene involving hTAFII68 and CHN from a t(9;17)(q22;q11.2) translocation in an extraskeletal myxoid chondrosarcoma. Attwooll, C., Tariq, M., Harris, M., Coyne, J.D., Telford, N., Varley, J.M. Oncogene (1999) [Pubmed]
  2. An in vitro enzymatic assay coupled to proteomics analysis reveals a new DNA processing activity for Ewing sarcoma and TAF(II)68 proteins. Guipaud, O., Guillonneau, F., Labas, V., Praseuth, D., Rossier, J., Lopez, B., Bertrand, P. Proteomics (2006) [Pubmed]
  3. hTAF(II)68, a novel RNA/ssDNA-binding protein with homology to the pro-oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II. Bertolotti, A., Lutz, Y., Heard, D.J., Chambon, P., Tora, L. EMBO J. (1996) [Pubmed]
  4. EWS, but not EWS-FLI-1, is associated with both TFIID and RNA polymerase II: interactions between two members of the TET family, EWS and hTAFII68, and subunits of TFIID and RNA polymerase II complexes. Bertolotti, A., Melot, T., Acker, J., Vigneron, M., Delattre, O., Tora, L. Mol. Cell. Biol. (1998) [Pubmed]
  5. Fusion of the NH2-terminal domain of the basic helix-loop-helix protein TCF12 to TEC in extraskeletal myxoid chondrosarcoma with translocation t(9;15)(q22;q21). Sjögren, H., Wedell, B., Meis-Kindblom, J.M., Kindblom, L.G., Stenman, G., Kindblom, J.M. Cancer Res. (2000) [Pubmed]
  6. The N-terminal domain of human TAFII68 displays transactivation and oncogenic properties. Bertolotti, A., Bell, B., Tora, L. Oncogene (1999) [Pubmed]
  7. TLS, EWS and TAF15: a model for transcriptional integration of gene expression. Law, W.J., Cann, K.L., Hicks, G.G. Briefings in functional genomics & proteomics. (2006) [Pubmed]
  8. Genomic structure of the human RBP56/hTAFII68 and FUS/TLS genes. Morohoshi, F., Ootsuka, Y., Arai, K., Ichikawa, H., Mitani, S., Munakata, N., Ohki, M. Gene (1998) [Pubmed]
  9. Cloning and mapping of a human RBP56 gene encoding a putative RNA binding protein similar to FUS/TLS and EWS proteins. Morohoshi, F., Arai, K., Takahashi, E.I., Tanigami, A., Ohki, M. Genomics (1996) [Pubmed]
  10. Stimulation of hTAFII68 (NTD)-mediated transactivation by v-Src. Lee, H.J., Kim, S., Pelletier, J., Kim, J. FEBS Lett. (2004) [Pubmed]
  11. Fusion of the EWS-related gene TAF2N to TEC in extraskeletal myxoid chondrosarcoma. Sjögren, H., Meis-Kindblom, J., Kindblom, L.G., Aman, P., Stenman, G. Cancer Res. (1999) [Pubmed]
  12. Cellular transformation of NIH3T3 fibroblasts by CIZ/NMP4 fusions. Corveleyn, A., Janssen, H., Martini, A., Somers, R., Cools, J., Marynen, P. J. Cell. Biochem. (2005) [Pubmed]
  13. Molecular genetic characterization of the EWS/CHN and RBP56/CHN fusion genes in extraskeletal myxoid chondrosarcoma. Panagopoulos, I., Mertens, F., Isaksson, M., Domanski, H.A., Brosjö, O., Heim, S., Bjerkehagen, B., Sciot, R., Dal Cin, P., Fletcher, J.A., Fletcher, C.D., Mandahl, N. Genes Chromosomes Cancer (2002) [Pubmed]
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